论文部分内容阅读
目的探讨荧光定量PCR(FQ-PCR)方法在疑似先天梅毒随访中的价值。方法 60例疑似先天梅毒病例在出生后的1月内,行血浆FQ-PCR、甲苯胺红不加热血清试验(TRUST)、梅毒螺旋体明胶凝集试验(TPPA)、19S-Ig M-TP-PA(只做一次)检测。在3、6、9、12、15、18月龄时进行血浆TP DNA、血清学TRUST和TPPA随访,当TRUST和TPPA均阴性时,则终止随访。采用SPSS18.0软件对检测结果进行统计分析。结果 60例疑似先天梅毒新生儿1月龄时,TRUST阳性的有51例,滴度均≤1:4;TPPA均阳性;19S Ig M-TPPA均阴性。疑似先天梅毒新生儿中,阳性病例的TPDNA拷贝量在l02~l03copies/m L。三种方法的检测结果在1、3、6、9月龄时的总阴转率有明显差异(P<0.05);在1、3月龄时FQ-PCR总转阴率最高,分别为75.00%、96.67%;6月龄时FQ-PCR和TRUST检测全部阴转;TPPA在1、3、6、9月龄总阴转率最低,12月龄全部阴转。结论 FQ-PCR定量检测血浆中TP DNA是一种敏感的方法,可应用于疑似先天梅毒的随访。
Objective To investigate the value of fluorescence quantitative PCR (FQ-PCR) in the follow-up of suspected congenital syphilis. Methods Sixty cases of suspected congenital syphilis were treated with plasma FQ-PCR, TRUST, TPPA and 19S-Ig M-TP-PA in one month after birth Only once) test. Plasma TP DNA, serological TRUST, and TPPA were followed up at 3, 6, 9, 12, 15, and 18 months of age. Follow-up was terminated when both TRUST and TPPA were negative. Using SPSS18.0 software to test the results of statistical analysis. RESULTS: Totally 51 cases of TRUST-positive neonates with congenital syphilis were found in 60 cases with titers ≤1: 4. Both TPPA and 19S Ig M-TPPA were negative. Among suspected neonates with congenital syphilis, the positive cases of TPDNA copy number l02 ~ l03copies / m L. The total negative conversion rates of the three methods were significantly different at 1, 3, 6 and 9 months of age (P <0.05). The total negative conversion rates of FQ-PCR at 1 and 3 months were the highest %, 96.67%. FQ-PCR and TRUST were all overcast at 6 months of age. TPPA had the lowest total negative conversion rate at 1, 3, 6 and 9 months of age, all at 12 months of age. Conclusion Quantitative detection of TP DNA in plasma by FQ-PCR is a sensitive method that can be used for the follow-up of suspected congenital syphilis.