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目的研究丁烯基苯酞在兔体内的药物动力学。方法生物样品采用液-液萃取法提取。HPLC测定兔血浆中的丁烯基苯酞,色谱柱为Kromasil C18柱(250 mm×4.6 mm,5μm),柱温25℃,甲醇-水(68:32)为流动相,流速1.0 ml.min-1,检测波长324 nm。结果血浆样品中丁烯基苯酞的线性范围为0.193~12.328μg.ml-1(r=0.9992),各浓度的方法回收率均大于80%,最低检测限为0.15μg.ml-1,日内、日间精密度均小于8%。给予兔一次性灌胃当归挥发油有效部位后,丁烯基苯酞在兔体内的药物动力学符合一级吸收二室模型,t1/2α=0.78±0.05 h,t1/2β=3.25±0.27 h。结论所建方法简便、快速,结果准确、可靠,可用于丁烯基苯酞的体内过程研究。丁烯基苯酞在兔体内吸收很快,消除也较快。
Objective To study the pharmacokinetics of butenylbenzoquinone in rabbits. Methods Biological samples were extracted using liquid-liquid extraction. Determination of butenylphenylhydrazine in rabbit plasma by HPLC with Kromasil C18 column (250 mm×4.6 mm, 5 μm), column temperature 25°C, mobile phase of methanol-water (68:32), flow rate 1.0 ml.min -1, detection wavelength 324 nm. Results The linear range of butenylbenzoquinone in plasma samples was 0.193~12.328μg.ml-1(r=0.9992). The recoveries of all concentrations were more than 80%, and the detection limit was 0.15μg.ml-1. The precision of daytime is less than 8%. The pharmacokinetics of butenylphenylhydrazine in rabbits was accorded with the first-order absorption two-compartment model when rabbits were given one-time intragastric administration of angelica volatile oil. t1/2α=0.78±0.05 h, t1/2β=3.25±0.27 h. Conclusion The established method is simple, rapid and accurate and reliable. It can be used for the in vivo study of butenylphenylhydrazine. Butylphthalide absorbs quickly in rabbits and is eliminated quickly.