论文部分内容阅读
目的:研究Toll样受体(Toll like receptors,TLR)4在脓毒症急性肺损伤(acute lung injury,ALI)大鼠肺内的动态表达情况。方法:32只Wistar大鼠随机分成对照组12只与ALI组20只。实验6,12,18,24h后分别处死大鼠。采用Real-time PCR测定肺组织TLR4 mRNA表达,免疫组织化学观察肺组织TLR4蛋白的表达情况。结果:ALI组与对照组比较,肺组织TLR4蛋白和TLR4 mRNA表达增加(P<0.01)。ALI组TLR4蛋白和TLR4 mRNA在盲肠结扎穿孔术术后逐渐增加至18h达高峰,后逐渐下降。结论:TLR4升高与脓毒症ALI的发生、发展密切相关。
Objective: To investigate the dynamic expression of TLR4 in the lung of septic rats with acute lung injury (ALI). Methods: Thirty-two Wistar rats were randomly divided into control group (n = 12) and ALI group (n = 20). Rats were sacrificed 6,12,18,24h after the experiment. The expression of TLR4 mRNA in lung tissue was detected by Real-time PCR and the expression of TLR4 protein in lung tissue was observed by immunohistochemistry. Results: Compared with the control group, the expressions of TLR4 and TLR4 mRNA in lung tissue of ALI group increased (P <0.01). In ALI group, TLR4 protein and TLR4 mRNA increased gradually to 18h after cecum ligation and perforation, and then decreased gradually. Conclusion: The elevated TLR4 is closely related to the occurrence and development of ALI in sepsis.