论文部分内容阅读
目的:分析儿童不分型先天性心脏病(CHD)致病的危险因素,探讨CHD与母亲亚甲基四氢叶酸还原酶(MTHFR)677C→T和血浆同型半胱氨酸(Hcy)的相关性。方法:采用病例对照研究方法,分析80对CHD患儿与对照儿父母所处的环境因素并检测其MTHFR677位点的多态性和血浆Hcy的水平,进行单因素及多因素Logistic回归分析。并且通过母子配对,分析MTHFR677位点多态性和血浆Hcy水平与CHD发生的相互关联。结果:①母孕早期发烧(OR=4.465)、孕期居住乡村(OR=2.234)、孕期吸烟环境(OR=20.529)、母子血浆Hcy水平增高(OR=3.342,OR=3.069)为不分型CHD的危险因素。②儿童与母亲MTHFR677位点基因型之间没有明显的关联,双方血浆Hcy水平亦无明显关联(P均>0.05)。③病例组与对照组MTHFR677位点基因型差异无统计学意义(P>0.05),病例组母子血浆Hcy水平均显著高于对照组相应值(P均<0.01)。结论:①母孕早期发烧、孕期居住乡村、孕期吸烟环境、母子血浆Hcy水平增高为不分型CHD的危险因素。②MTHFR基因677C→T对血浆Hcy水平影响有待于进一步研究。③在CHD发生上,母子MTHFR基因677位点基因型之间和血浆Hcy水平之间均没有相关性。
OBJECTIVE: To analyze the risk factors of children with undifferentiated congenital heart disease (CHD) and to explore the relationship between CHD and maternal methylenetetrahydrofolate reductase (MTHFR) 677C → T and plasma homocysteine (Hcy) Sex. Methods: A case-control study was conducted to analyze the environmental factors in 80 pairs of CHD children and controls and to detect the MTHFR677 polymorphism and plasma Hcy levels. Univariate and multivariate logistic regression analysis were performed. And maternal-paired analysis of MTHFR677 polymorphism and plasma Hcy levels and CHD occurred correlation. Results: (1) There was a significant difference between the first trimester females and the second females (OR = 4.465, OR = 2.234, OR = 20.529, OR = 3.342, OR = 3.069) Risk factors. There was no significant correlation between MTHFR677 locus genotypes in children and mothers, nor did there be any significant correlation between plasma homocysteine levels (P> 0.05). ③ The genotypes of MTHFR677 in case group and control group had no significant difference (P> 0.05). The plasma Hcy levels in both cases were significantly higher than those in control group (all P <0.01). Conclusions: ①In the first trimester of pregnancy, there is a risk of fever, dwelling country during pregnancy, smoking environment during pregnancy, and increasing plasma Hcy level in mother and child as the non-typed CHD. ② MTHFR gene 677C → T on plasma Hcy levels need further study. ③ In the occurrence of CHD, there was no correlation between 677 genotypes of maternal and MTHFR gene and plasma Hcy level.