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肌苷(9-D核糖次黄嘌岭)为核酸类药物。次黄嘌岭是生产过程中的分解产物,对肌苷的纯度有影响。为提供快速简便的检查方法,本实验采用纸层析法,人为的将次黄嘌呤作为杂质混入肌苷溶液中,并选用八种展开系统进行对照比较,最后选出需时短、分离效果好的展开剂。并作最小检出量的试验。实验部分 (一)材料和仪器 1.新华1号层析滤纸 2.层析用微量吸管 3.254nm紫外分析仪(上海科艺光学仪器厂)
Inosine (9-D ribose hypoxanthine) is a nucleic acid drug. Sub-xuan purine is the production of decomposition products, on the purity of inosine impact. In order to provide a quick and easy inspection method, paper chromatography was used in this experiment, and hypoxanthine was artificially mixed into inosine solution as an impurity, and eight kinds of unfolding systems were selected for comparison. Finally, short time was needed and separation effect was good Of the agent. And for the minimum amount of test. Experimental part (a) materials and instruments 1 Xinhua No. 1 chromatography filter 2 chromatography with micropipette 3.254nm UV analyzer (Shanghai Branch Arts Optical Instrument Factory)