目的　观察减毒麻疹病毒与拉米夫定 (3TC)联合用药在鸭体内抗乙型肝炎病毒的作用。方法　以感染鸭乙型肝炎病毒 (DHBV)的广州麻鸭为实验动物模型 ,在感染后分别给予口服3TC、肌注麻疹疫苗和联合使用麻疹疫苗与 3TC ,并以无环鸟苷 (ACV)口服作为用药对照组 ,肌注生理盐水作为病毒对照组。于给药前 (T0 ) ,给药第 5天 (T5) ,第 10天 (T10 )及停药第 3天 (T13)留取血清 ,采用斑点杂交方法测定DHBVDNA。结果　 3TC组在T5和T13 两点病毒量略有下降 ,但与T0 比较 ,差异均无显著性 (P >0 0 5 ) ;麻疹疫苗组在给药早期病毒量无下降 ,T13 点时病毒量与T0 比较有显著下降 (P <0 0 1) ,与病毒对照组的同期比较 ,差异也有显著性 (P <0 0 5 ) ;麻疹疫苗与 3TC联合使用组在给药早期 (T5)出现病毒量明显下降 (与T0 比较 ,P <0 0 1) ,其A值与有确定疗效的ACV组T5比较也无差异 (P >0 0 5 ) ,停药第 3天时的病毒量与给药前及病毒对照组同期比较 ,差异均有显著性 (P <0 0 1,P <0 0 5 )。结论　减毒麻疹病毒与 3TC联合给药较单独给药对DHBV DNA具有更早而持久的抑制作用 Objective To observe the effect of combination of attenuated measles virus and lamivudine (3TC) on hepatitis B virus in duck. Methods Guangzhou ducklings infected with duck hepatitis B virus (DHBV) were used as experimental animal models. After the infection, oral administration of 3TC, intramuscular measles vaccine and measles vaccine combined with 3TC were given respectively. Oral administration of acyclovir (ACV) As a control group, intramuscular saline as a virus control group. Serum was collected before administration (T0), on the fifth day (T5), on the tenth day (T10) and on the third day (T13). DHBVDNA was determined by dot blot hybridization. Results The viral load in 3TC group decreased slightly at T5 and T13, but no significant difference was found between TTC and T0 (P> 0.05) (P <0.01), compared with the same period of the virus control group, the difference was also significant (P <0 05); the combined use of measles vaccine and 3TC in the early administration (T5) of the virus (P <0.01) compared with T0. There was no significant difference between A value and T5 in definite ACF group (P> 0.05) And the virus control group over the same period, the differences were significant (P <0.01, P <0 05). Conclusions The combined administration of attenuated measles virus and 3TC has an earlier and longer-lasting inhibitory effect on DHBV DNA than single administration