论文部分内容阅读
目的同步观察高血糖对离体施万细胞增殖能力和神经生长因子(NGF)合成水平的影响,探讨周围神经再生时糖尿病性损伤的可能机制。方法采用Brockes改良法从新生Wistar乳鼠坐骨神经组织分离纯化施万细胞。采用3H-TdR掺入法检测离体施万细胞的增殖能力,用ELISA法测定离体施万细胞NGF的合成水平。结果经ABC法染色计数,抗S100免疫组化染色着色细胞的阳性率为(92±2)%,表明采用该法鉴定显示着色细胞为表达特异性抗原S100的施万细胞。高血糖组与对照组比较,细胞增殖能力和NGF合成显著受到抑制(P<0.01)。结论离体施万细胞实验性高血糖损伤所致的增殖能力下降可能与高血糖状态下施万细胞的NGF合成减少密切相关。
Objective To observe the effect of hyperglycemia on the proliferation and the synthesis of nerve growth factor (NGF) in vitro and to explore the possible mechanism of diabetic nephropathy. Methods Schwann cells were isolated and purified from the sciatic nerve of newborn Wistar rats using Brockes method. The proliferation of Schwann cells was detected by 3H-TdR incorporation method. The synthesis of NGF in vitro was determined by ELISA. Results The positive staining rate of stained cells by anti-S100 immunohistochemistry was (92 ± 2)% by ABC staining method, which indicated that this method was used to identify Schwann cells that showed the stained cells as S100 expressing specific antigen. Hyperglycemia group and control group, cell proliferation and NGF synthesis was significantly inhibited (P <0.01). Conclusion The decrease of proliferative capacity induced by experimental hyperglycemia in vitro may be closely related to the decrease of NGF synthesis in Schwann cells under hyperglycemia.