本实验以自然温度(-22℃)冻融蟾蜍心脏。冷冻50分钟后,以温水30℃复温,1—2分钟后,蟾蜍复苏。经组织学常规处理,HE染色,光镜观察:结果发现,整体和开胸腹冻融的心肌组织结构完整,心肌纤维、心肌细胞核及闰盘清楚可见。离体冻融的心脏心肌纤维部分断裂和透明玻璃样变,心肌细胞核增大。上述结果表明:在体蟾蜍心脏可以在—22℃冷冻至少50分钟。蟾蜍在30℃温水中复温,1—2分钟后复苏,心肌组织有活力和功能。 In this experiment, the heart was frozen and thawed at natural temperature (-22°C). After freezing for 50 minutes, rewarming was performed with warm water at 30°C. After 1-2 minutes, the sputum recovered. After routine histological examination, HE staining, and light microscopy, it was found that the whole and open thoracoabdominal frozen and thawed myocardial tissues were intact, and myocardial fibers, myocardial cell nucleus, and sacral discs were clearly visible. In vitro freeze-thaw of myocardial fibrosis partially ruptured and hyalinized hyaline, myocardial nuclei increased. The above results show that the heart of the body can be frozen at -22°C for at least 50 minutes.蟾蜍 Rewarming in warm water at 30°C, recovery after 1-2 minutes, myocardial tissue has vitality and function.