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目的：探讨分子杂交技术检测免疫球蛋白基因重排的意义。方法：采用Ｊ＿Ｈ和Ｃ＿λ探针，经Ｓｏｕｔｈ－ｅｒｎ印迹法检测１２例确诊或疑诊血液系统肿瘤患者的２０份淋巴结、血和骨髓细胞的Ｉｇ基因重排。结果：发现Ｂ－ＮＨＬ、ＣＬＬ、ＨＣＬ和ＭＭ均有不同于胚系的重排模式，而淀粉样变性、ＣＭＬ、Ｔ－ＡＬＬ和鼻咽癌为胚系模式。结论：克隆性Ｉｇ基因重排仅见于Ｂ细胞，可作为Ｂ细胞克隆性增殖的证据，用于Ｂ细胞恶性增生性疾病的诊断，也可以用于分析Ｂ细胞肿瘤的克隆来源。
Objective: To explore the significance of molecular hybridization to detect immunoglobulin gene rearrangements. Methods: The Ig gene rearrangements of 20 lymph nodes, blood and bone marrow cells from 12 patients diagnosed or suspected hematological malignancies were detected by South-ern blotting using J_H and C_λ probes. RESULTS: B-NHL, CLL, HCL and MM were found to differ from the germline rearrangement pattern, whereas amyloidosis, CML, T-ALL and nasopharyngeal carcinoma were germline patterns. CONCLUSIONS: Clonal Ig gene rearrangements are found only in B cells and can be used as evidence for the clonal proliferation of B cells for the diagnosis of B-cell malignant proliferative diseases and for the analysis of the clonal origin of B-cell tumors.