The dynamic changes of protein polypeptide in endosperms of Gingkgo biloba seeds during seed germination were studied by SDS-PAGE and two-dimensional gel electrophoresis (2-DE). The results showed that 80 kinds of protein spots in endosperms of Gingkgo biloba were clear observed in the 2-DE spectrum. Protein molecular weights were in the range of 26-52kD, and their isoelectric points were in the range of 5.8-7.8. In the course of seed germination, 13 kinds of proteins were degraded, and 13 kinds of proteins were synthesized; 7 kinds of proteins with different molecular weights and isoelectric points of 35kD/pI6.8, 31kD/pI6.8, 29kD/pI6.8, 33kD/pI6.6, 33kD/pI 6.4, 34kD/ pI7.7 and 31kD/pI7.7 were identified primarily as vegetative storage proteins (VSPs). The dynamic changes of protein polypeptide in endosperms of Gingko biloba seeds during seed germination were studied by SDS-PAGE and two-dimensional gel electrophoresis (2-DE). The results showed that 80 kinds of protein spots in endosperms of Gingko biloba were clear observed in the 2-DE spectrum. Protein molecular weights were in the range of 26-52 kD, and their isoelectric points were in the range of 5.8-7.8. In the course of seed germination, 13 kinds of proteins were degraded, and 13 kinds of 7 kinds of proteins with different molecular weights and isoelectric points of 35 kD / and 31kD / pI7.7 were identified as vegetative storage proteins (VSPs).