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体内移植研究表明,骨髓来源的干细胞具有向成熟的肝细胞分化的能力。然而,成体干细胞向肝细胞诱导分化的效率、分化细胞的功能状态、微环境对细胞分析的影响等均存在许多疑问。以高效、稳定表达肝细胞生长因子的肝星状细胞株CFSC/HGF作为饲养细胞,成功地将大鼠骨髓来源的Thy-1+β2M-细胞(bone marrow derived Thy-1+β2M- cells,BDTCs)诱导为肝细胞,经RT-PCR和免疫荧光化学检测证实,该条件下诱导后的BDTCs表达幼稚或成熟肝细胞特异性的基因,分化细胞具有成熟肝细胞特有的靛青绿摄取排泌、氨基代谢和白蛋白分泌的功能,提示肝细胞生长因子、肝脏非实质细胞及其产生的细胞外基质为骨髓干细胞向肝细胞分化提供了适宜的微环境。这为以骨髓干细胞为基础的再生医学在终末期肝病治疗中的应用提供了理论和技术上的支持。
In vivo transplantation studies have shown that bone marrow-derived stem cells have the ability to differentiate into mature hepatocytes. However, the efficiency of adult stem cells to induce differentiation into hepatocytes, the functional status of differentiated cells, the impact of microenvironment on cellular analysis, and many others remain problematic. The bone marrow derived Thy-1 + β2M-cells (BDTCs) were successfully transfected into rat hepatic stellate cell line CFSC / HGF with high efficiency and stable expression of hepatocyte growth factor as feeder cells ) Were induced into hepatocytes. RT-PCR and immunofluorescence staining confirmed that BDTCs expressed naive or mature hepatocyte-specific genes under these conditions. The differentiated cells had the characteristics of indigo green uptake and excretion of mature hepatocytes, Metabolism and albumin secretion function, suggesting that hepatocyte growth factor, liver non-parenchymal cells and the extracellular matrix produced by the bone marrow stem cells provide a suitable microenvironment for hepatocyte differentiation. This provides theoretical and technical support for the application of bone marrow stem cell-based regenerative medicine in the treatment of end-stage liver disease.