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目的：研究细胞粘附分子（ｃｅｌｕｌａｒａｄｈｅｓｉｏｎｍｏｌｅｃｕｌｅｓ，ＣＡＭｓ）在炎症角膜中的表达，探讨其在角膜炎症发生机制中的作用。方法：采用冰冻切片免疫组织化学染色方法检测了１８只炎症角膜、３只正常角膜和２只圆锥角膜组织中细胞间粘附分子１（ｉｎｔｅｒｃｅｌｕｌａｒａｄｈｅｓｉｏｎｍｏｌｅｃｕｌｅ１，ＩＣＡＭ１）、人类白细胞抗原ＤＲ（ｈｕｍａｎｌｅｕｋｏｃｙｔｅａｎｔｉｇｅｎＤＲ，ＨＬＡＤＲ）、ＣＤ１１ａ、ＣＤ１１ｂ、ＣＤ１８、ＣＤ２和ＣＤ２０的表达及变化。结果：正常角膜中，ＩＣＡＭ１主要表达于角膜缘血管内皮细胞，基质细胞、角膜内皮细胞中仅有微弱表达或不表达。炎症角膜中，基质细胞、内皮细胞和血管内皮细胞的ＩＣＡＭ１表达增强，尤其在炎症细胞浸润处最为显著；原不表达ＩＣＡＭ１的上皮细胞亦出现异常表达；ＩＣＡＭ１和ＨＬＡＤＲ常共同表达于同一部位，炎症明显组角膜ＩＣＡＭ１和ＨＬＡＤＲ的表达水平明显高于炎症轻微组；炎症浸润细胞中表达ＬＦＡ１（ＣＤ１１ａ／ＣＤ１８）的细胞较表达Ｍａｃ１（ＣＤ１１ｂ／ＣＤ１８）的细胞多；浸润淋巴细胞以Ｔ（ＣＤ２）淋巴细胞为主；属非炎症性病变的圆锥角膜中Ｉ?
Objective: To study the expression of cell adhesion molecules (CAMs) in the cornea of inflamed cornea and to explore their roles in the pathogenesis of corneal inflammation. Methods: Frozen section immunohistochemical staining was used to detect the expression of intercellular adhesion molecule-1 (ICAM-1), interleukin-1 (HLA-DR), and leukocyte antigen DR in 18 inflammatory corneas, 3 normal corneas and 2 keratoconus Human leukocyte antigenDR, HLA DR), CD11a, CD11b, CD18, CD2 and CD20 expression and changes. Results: In normal cornea, ICAM1 was mainly expressed in corneal limbal vascular endothelial cells, stromal cells and corneal endothelial cells with only weak expression or no expression. Inflammation of the cornea, stromal cells, endothelial cells and vascular endothelial cells ICAM 1 expression increased, especially in inflammatory cell infiltration at the most significant; the original does not express ICAM 1 epithelial cells also appear abnormal expression; I CAM 1 and HLA DR often co-expressed in the same location, the group of inflammation significantly corneal ICAM 1 and HLA-DR expression was significantly higher than the inflammation group; inflammatory infiltration of cells expressing LFA 1 (CD11a / CD18) cells more expression Mac 1 (CD11b / CD18) cells; infiltrating lymphocytes to T (CD2) lymphocytes; non-inflammatory lesions in the keratoconus I?