目的: 建立一种利用拉曼技术快速测定注射用更昔洛韦的方法.“,”Objective: To establish a Raman spectroscopy method for the determination of ganciclovir for injection.Methods: Raman spectroscopy was used to determine the content of ganciclovir for injection and compared with that determined by an HPLC method.The conditions of Raman spectroscopy were as follows: the optical fiber automatic measurement mode was used, the focal length of the probe was 5 mm, the excitation light source was 785 nm, the resolution was 4 cm-1, the test time was 100 s, the laser output power was 400 mW, the scanning range was 3 000-200 cm-1, and the operating temperature of detector was-50℃.Results: Ganciclovir had a good linear relationship with peak area at 1 475 cm-1 within the concentration range of 2.5-50.0 mg · ml-1 (r=0.998 0).The average recovery was 99.47%(RSD=1.02%, n=9).Conclusion: The method is fast and simple, and can be used for the rapid determination of ganciclovir for injection.