论文部分内容阅读
实验选择黄嘌呤──黄嘌呤氧化酶作为系统，反应在Ｏ２存在的条件下，废物黄嘌呤发生催化氧化，产生超氧自由基（Ｏ２．－），由２．６－二氯靛酚钠试剂检测。通过运用Ｍｉｃｈａｅｌｉｓ反应机理处理，最终得到米氏常数（ＫＭ）。Ｃｕ２＋能抑制黄嘌呤氧化酶的作用效果，其抑制作用可通过ｖ０－［Ｃｕ２＋］曲线看出。
The xanthine-xanthine oxidase was chosen as a system to catalyze the oxidation of xanthine in the presence of O2 in the presence of O2 to produce superoxide radical (O2.-), which was prepared from 2.6-dichloroindophenoxide sodium reagent Detection. By using the Michaelis reaction mechanism, the Michaelis constant (KM) is finally obtained. Cu2 + can inhibit the effect of xanthine oxidase, the inhibition can be seen by v0- [Cu2 +] curve.