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本试验在CO2加富,气温为30℃～35℃条件下,将空气湿度设置为95%±5%(L1),75%±5%(L2),55%±5%(L3)3个处理,研究了空气湿度调控对大棚嫁接黄瓜逆境生理的影响。结果表明:随着处理时间的延长,3个处理叶片中的渗透调节物质、丙二醛含量和保护酶活性均呈增加趋势,细胞质膜透性L2处理和L3处理增加,L1处理降低;随着处理空气湿度的降低,黄瓜叶片中的渗透调节物质、丙二醛含量、保护酶活性和细胞质膜透性均呈降低趋势。在处理第24d时,L1比L2、L3的可溶性蛋白含量降低了4.84%、8.18%,脯氨酸含量降低了31.83%、36.77%,可溶性糖含量降低了27.74%、32.52%、丙二醛含量降低了30.34%、32.38%,超氧化物歧化酶(SOD)活性降低了29.45%、37.75%,过氧化氢酶(CAT)活性降低了17.41%、17.41%,过氧化物酶(POD)活性降低了9.14%、44.87%;细胞质膜透性降低了80.75%和80.76%。说明大棚内在CO2加富条件下,在较高的空气湿度环境中,嫁接黄瓜遭受的逆境胁迫较小。进一步证明了亚高温管理大棚可提高嫁接黄瓜抵御逆境胁迫的能力,降低逆境胁迫的程度,缓解逆境对植株的伤害。
In this experiment, air humidity was set to 95% ± 5% (L1), 75% ± 5% (L2) and 55% ± 5% (L3) under the condition of CO2 enrichment and temperature of 30 ℃ ~ 35 ℃ The effects of air humidity regulation on the adversity physiology of grafted cucumbers in greenhouse were studied. The results showed that with the prolongation of treatment time, the osmotic adjustment substances, malondialdehyde (MDA) content and the activity of protective enzymes increased in the three treatment leaves. The permeability of the plasma membrane permeability L2 and L3 increased and the content of L1 decreased The decrease of air humidity, osmotic adjustment substance, malondialdehyde content, protective enzyme activity and cytoplasmic membrane permeability in cucumber leaves all showed a decreasing trend. At the 24th day after treatment, the contents of soluble protein in L1 and L2 and L3 decreased by 4.84% and 8.18%, the proline content decreased by 31.83% and 36.77%, the soluble sugar content decreased by 27.74% and 32.52% Decreased by 30.34% and 32.38%, SOD activity decreased by 29.45% and 37.75%, CAT activity decreased by 17.41% and 17.41%, POD activity decreased 9.14% and 44.87% respectively. The plasma membrane permeability decreased by 80.75% and 80.76%. The results showed that under the conditions of CO2 enrichment in greenhouse, grafted cucumber suffered less stress in higher air humidity environment. It was further proved that the management of greenhouse can improve the ability of grafted cucumber to resist stress, reduce the degree of stress and alleviate the harm to plant by adversity.