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目的观察β-连环蛋白在转染p120ctn同工蛋白3A的肝癌细胞中的表达变化;为通过这一途径实施肿瘤的基因治疗提供实验依据。方法对BEL-7404人肝癌细胞进行稳定转染p120ctn同工蛋白3A,然后用激光共聚焦显微镜和免疫印迹技术,检测β-连环蛋白在细胞中的表达情况,同时检测细胞黏附、细胞迁移以及增殖能力的改变。结果肝癌细胞转染p120ctn同工蛋白3A后,促进了β-连环蛋白与上皮钙黏蛋白的结合,表现为其与上皮钙黏蛋白的沉淀量增加,在细胞膜上的表达增强,在细胞核中的表达趋向减弱;细胞黏附能力增强,迁移及增殖能力下降。结论转染p120ctn同工蛋白3A,能弱化β-连环蛋白在细胞核中的表达量,削弱了BEL-7404人肝癌细胞的恶性行为,是调控β-连环蛋白癌基因表达的有效途径之一。
Objective To observe the expression of β-catenin in hepatocellular carcinoma cells transfected with p120ctn isoenzyme 3A, and to provide experimental evidence for gene therapy of tumor through this pathway. Methods Stable transfection of p120ctn isoform 3A on BEL-7404 human hepatocellular carcinoma cells was carried out. The expression of β-catenin in cells was detected by laser confocal microscopy and Western blotting. Meanwhile, cell adhesion, cell migration and proliferation Ability to change. Results The hepatoma cells transfected with p120ctn isoenzyme 3A promoted the binding between β-catenin and e-cadherin, which showed an increase in the deposition of e-cadherin and increased expression in the cell membrane, The expression tended to be weakened; cell adhesion ability enhanced, migration and proliferation decreased. CONCLUSION: Transfection of p120ctn isoenzyme 3A can attenuate the expression of β-catenin in the nucleus and impair the malignant behavior of BEL-7404 human hepatoma cells. It is one of the effective ways to regulate the expression of β-catenin.