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目的:探讨红花黄色素对饮食诱导肥胖小鼠的体脂、脂肪肝及胰岛素抵抗的影响。方法:4周龄雄性C57BL/6小鼠喂以高脂饮食8周,建立肥胖模型,随后进行6周的腹腔注射红花黄色素(100 mg·kg~(-1)·d~(-1))干预。实验终点行腹腔葡萄糖耐量试验(IPGTT)及胰岛素糖耐量(ITT)试验,小鼠处死后,检测体脂、血脂等代谢参数,并采集附睾脂肪及肝脏组织,行HE染色,定量脂肪细胞面积、观察肝脏脂肪变性程度。结果:红花黄色素可显著降低肥胖小鼠的体质量、体脂含量、脂肪细胞面积、肝脏重量及血脂水平(P<0.05),并且明显减轻肥胖小鼠肝脏脂肪变性程度,同时,IPGTT及ITT实验显示红花黄色素可显著改善肥胖小鼠的糖耐量异常,增强胰岛素的敏感性(P<0.05)。结论:红花黄色素可显著降低饮食诱导肥胖小鼠的体脂含量,减轻脂肪肝,改善糖耐量异常及胰岛素抵抗。
Objective: To investigate the effects of safflor yellow on body fat, fatty liver and insulin resistance in diet-induced obese mice. Methods: Four-week-old male C57BL / 6 mice were fed a high-fat diet for 8 weeks to establish a model of obesity, followed by 6 weeks of intraperitoneal injection of safflower yellow (100 mg · kg -1 · d -1 )) Intervention. At the end of the experiment, IPGTT and ITT test were performed. After the mice were sacrificed, the metabolic parameters of body fat and lipids were measured. Epididymal fat and liver tissues were collected. HE staining was used to determine the area of adipocytes, Observe the degree of hepatic steatosis. Results: The safflower yellow pigment could significantly reduce body weight, body fat content, fat cell area, liver weight and blood lipid level in obese mice (P <0.05), and significantly reduce the degree of hepatic steatosis in obese mice. Meanwhile, IPGTT and ITT experiments show that safflower yellow pigment can significantly improve the obese mice glucose tolerance abnormalities, increased insulin sensitivity (P <0.05). Conclusion: Safflower yellow pigment can significantly reduce body fat content in diet-induced obese mice, reduce fatty liver, improve glucose tolerance and insulin resistance.