迄今已经发现了多种细胞液腺苷酸环化酶(Ac)激活因子。它们来源不同,其性质各异。本文利用从大白鼠肝脏提取的一种细胞液Ac激活因子与大鼠或人血影细胞(“ghost”)建立了一种重组系统,能灵敏地测出大鼠和人红细胞血影细胞及其细胞骨架的Ac活力;同时研究了这种活性因子的物理参数、动力学特征及激活Ac的可能机制。灌流后的大鼠肝细胞用超声波破碎,或将大鼠肝脏作成1:3组织匀浆,经20,000×g,0～4℃离 To date, a variety of cellular adenylate cyclase (Ac) activators have been discovered. They come from different sources and have different properties. In this paper, a recombinant system was established by using a cytokine Ac activator extracted from rat liver to establish a recombinant system with rat or human ghost cells (“ghost”), which can sensitively detect rat and human erythrocyte ghost cells and their Ac activity of cytoskeleton was also studied. The physical parameters, kinetic characteristics and possible mechanisms of Ac activation were also studied. Perfused rat liver cells were sonicated or rat livers were made into 1: 3 tissue homogenate and subjected to 20,000 × g, 0-4 ° C