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以羽毛藻为研究材料,利用CO_2培养箱通入不同CO_2浓度的空气进行培养,探究在高浓度CO_2和高浓度硝氮条件下羽毛藻生理生化的变化。结果表明,高浓度CO_2或硝氮都能显著促进藻体的生长,高碳高氮下藻体的相对生长率比低碳低氮组高出63.77%。另外,高浓度CO_2会使藻体中的叶绿素、类胡萝卜素、藻红蛋白、藻蓝蛋白、可溶性蛋白和可溶性碳水化合物的含量下降,而使硝酸还原酶活性升高,其中明显的表现为高碳低氮组叶绿素a含量比低碳低氮组降低了39.01%,高碳高氮组比低碳高氮组降低了49.26%,相应类胡萝卜素高碳组分别降低了47.62%和43.24%,增加硝氮浓度能够提高藻体中色素含量,可溶性蛋白和可溶性碳水化合物的含量;在高浓度硝氮下,提高CO_2浓度能使硝酸盐和磷酸盐的吸收率显著升高。由此得知大气CO_2浓度升高或海水富营养化会促进羽毛藻的生长,并且这两个条件的改变会对羽毛藻在生化组成和营养盐吸收方面产生深刻的影响。
Using Plumeria as the research material, CO 2 incubator was used to introduce air with different concentrations of CO 2 to study the changes of physiological and biochemical characteristics of Plumeria under high concentration of CO 2 and high concentration of nitrate nitrogen. The results showed that both high concentration of CO 2 and nitrate nitrogen could significantly promote the growth of algae. The relative growth rate of algal biomass under high carbon and high nitrogen was 63.77% higher than that of low carbon and low nitrogen. In addition, high concentrations of CO 2 decreased the content of chlorophyll, carotenoids, phycoerythrin, phycocyanin, soluble protein and soluble carbohydrates in the algae, and increased the nitrate reductase activity, of which the obvious was high The content of chlorophyll a in carbon-nitrogen low-nitrogen group decreased by 39.01%, the carbon-nitrogen high-nitrogen group reduced by 49.26% and the corresponding carotenoid-carbon group decreased by 47.62% and 43.24%, respectively, Increasing the concentration of nitrate could increase the content of pigment, soluble protein and soluble carbohydrate in the algae. Under the high concentration of nitrate, increasing the concentration of CO 2 could significantly increase the nitrate and phosphate absorption. It is concluded that the increase of atmospheric CO 2 concentration or seawater eutrophication can promote the growth of feather algae, and the changes of these two conditions will have a profound impact on the biochemical composition and nutrient absorption of feather algae.