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目的:探讨重组人骨形态发生蛋白-7(rhBMP-7)对NIH3T3增殖和骨向分化的影响。方法:向培养的成纤维细胞NIH3T3中加入不同浓度的rhBMP-7,观察NIH3T3增殖、碱性磷酸酶(ALP)活性和骨钙素(OCN)含量的变化。结果:rh-BMP-7在一定浓度范围内可以明显促进NIH3T3细胞增殖、提高ALP活性与OCN水平。结论:rhBMP-7可以刺激NIH3T3细胞增殖,诱导NIH3T3细胞向成骨细胞表型分化。
Objective: To investigate the effect of recombinant human bone morphogenetic protein-7 (rhBMP-7) on the proliferation and differentiation of NIH3T3 cells. Methods: Different concentrations of rhBMP-7 were added into cultured fibroblasts NIH3T3 to observe the changes of NIH3T3 proliferation, alkaline phosphatase (ALP) activity and osteocalcin (OCN) content. Results: rh-BMP-7 could significantly promote the proliferation of NIH3T3 cells and increase the activity of ALP and the level of OCN in a certain concentration range. Conclusion: rhBMP-7 can stimulate the proliferation of NIH3T3 cells and induce the differentiation of NIH3T3 cells into osteoblasts.