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目的:研究黄芪皂苷IV(XGA)对缺血大鼠心肌钙运转和心功能的影响.方法:84只Wistar大鼠分为3组:对照组(12只);缺血组(12只),皮下注射异丙肾上腺素(30 mg/kg);XGA组(60只),皮下注射异丙肾上腺素后给予XGA.测定大鼠血流动力学指标、心脏功能和心肌细胞内外钙的变化,观察XGA对缺血大鼠心肌钙运转和心功能的量-效和时-效关系.结果:给予XGA后缺血大鼠心功能和血流动力学明显改善,随着XGA剂量的增加和XGA作用时间的延长,缺血大鼠的心输出量、心率、每博量、平均动脉压、动脉收缩压、左心室搏出功、左室和右室收缩末期压力和右心室的+dp/dt逐渐恢复到对照组水平;给予XGA后心肌细胞游离钙和心肌组织总钙与缺血组比较明显下降,而红细胞膜钙泵活性较缺血组明显增加,但心肌细胞游离钙和心肌组织总钙下降的幅度及红细胞膜钙泵活性增加的幅度并没有随XGA剂量的增加而增加;随XGA作用时间的延长,心肌细胞游离钙逐渐下降.结论:XGA能够明显改善缺血大鼠的心功能,减少心肌细胞内过多的钙积聚是其作用机制之一.
Objective: To study the effects of astragaloside IV (XGA) on cardiac calcium transport and cardiac function in ischemic rats. Methods: Eighty-four Wistar rats were divided into three groups: control group (12 rats) and ischemic group (12 rats). Subcutaneous injection of isoproterenol (30 mg/kg); XGA group (60 rats) given XGA after subcutaneous injection of isoproterenol. Determination of hemodynamic parameters, cardiac function, and intracellular and extracellular calcium in rats. Effect of XGA on Myocardium Calcium Translocation and Cardiac Function in Ischemic Rats: A Quantitative-Effective and Time-Effective Relationship. Results: After XGA administration, cardiac function and hemodynamics were significantly improved in ischemic rats, with the increase of XGA dose and XGA effect. Time prolonged, cardiac output, heart rate, each bolus volume, mean arterial pressure, arterial systolic pressure, left ventricular stroke work, left ventricular and right ventricular end-systolic pressure, and right ventricular + dp/dt in ischemic rats gradually Recovered to the control group; after the administration of XGA, the free calcium in myocardial cells and total calcium in myocardial tissue decreased significantly compared with the ischemic group, but the activity of calcium pump in red blood cell membrane increased significantly compared with that in ischemic group, but the free calcium in cardiomyocytes and total calcium in myocardial tissue decreased. The magnitude of the increase in the magnitude of the red blood cell membrane calcium pump activity did not increase with the XGA dose. Plus; XGA prolonged action over time, decreased intracellular calcium in cardiac Conclusion: XGA is possible to significantly improve cardiac function in ischemic rats, reduce the excessive accumulation of intracellular calcium is one of the mechanisms.