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本研究旨在明确纤维蛋白胶(Tissel)输精管吻合术后大鼠的复育率是否比得上传统显微外科缝合吻合术。为了摸拟近似人类输精管切除术后的变化,20只体重250～300g雄性Sprague-Dawley大鼠于输精管切除术后2周作延迟输精管吻合术。此时睾丸端输精管直径大约为精囊端的2～2.5倍。受试大鼠均分二组,Ⅰ组作传统显微外科输精管吻合,Ⅱ组为延迟纤维蛋白胶粘合输精管吻合。两组均用腹膜内戊巴比妥(50mg/kg)麻醉,腹中线切口,游离输精管两断端,取睾丸端管腔液,镜检证实有精子后,Ⅰ组采用2层输精管显微外科端端吻合。粘膜和浆肌膜用10-0单丝尼龙线各缝4针。Ⅱ组在6、12点位置用10-0尼龙线透壁各缝合一针,将纤维蛋白复合物于吻合部周边作环形涂布。经3～5分钟封固剂粘合后,松开输精管夹。纤维蛋白封固剂由4种成分组成:浓缩纤维蛋白原(120mg/ml),纤维蛋白溶解抑制剂(抑肽酶3000KIU/ml),干燥纤维蛋白酶500μ(ml)以及氯化钙(40μm/ml)。预热至37℃,将纤维蛋白原和干燥纤维蛋白酶分别置于抑肽酶和氯化钙中再组成。用两个独立的注射器分别抽吸两种溶液,再将注射器截入一个可
The purpose of this study was to determine if the fertility rate of rats after staple anastomosis of fibrin glue is comparable to that of conventional microsurgical suture anastomosis. To simulate post-vasectomy changes, 20 male Sprague-Dawley rats weighing 250-300 g were delayed vasectomy 2 weeks after vasectomy. Testicular vas deferens at this time the diameter of about 2 to 2.5 times the seminal vesicle side. The rats were equally divided into two groups. Group I received conventional microsurgical vas deferens anastomosis. Group II was delayed fibrin glue-mounted vas deferens anastomosis. Both groups were anesthetized by intraperitoneal injection of pentobarbitone (50mg / kg), incision was made on the midline, and both ends of the vas deferens were removed. After the spermatozoa were obtained by microscopic examination, the rats in group I were treated with 2-layer vas deferens microsurgery End anastomosis. Mucosa and pulp muscle membrane with 10-0 monofilament nylon thread each stitch 4 needles. Group Ⅱ at 6, 12 o’clock position with 10-0 nylon thread through the suture of a needle, the fibrin compound in the anastomosis around the ring for coating. After 3 to 5 minutes after the adhesive bonding agent, release the vas deferens clip. Fibrin sealant consists of four components: concentrated fibrinogen (120 mg / ml), fibrinolytic inhibitor (aprotinin 3000 KIU / ml), dry fibrinase 500 μ (ml) and calcium chloride ). Preheated to 37 ℃, fibrinogen and dry fibrin were placed in aprotinin and calcium chloride re-composition. Two separate syringes were used to aspirate the two solutions separately and the syringes were cut into one