论文部分内容阅读
甘薯(Ipomoeabatatas)抗病品种"湘薯6号"和感病品种"新种花183"经薯瘟病原细菌(PseudomonassolanacoarumE.F.Smith)的诱导,90%甲醇提取后,用乙酸乙酯萃取和SephadexLH-20柱层析分离纯化得到4个吸收峰.经抑菌试验检测出"湘薯6号"的抑菌活性区在第2峰,而"新种花183"的抑菌活性区在第3峰.硅胶G254薄板层析法鉴定该提取物可能是植保素.紫外光谱、红外光谱分析表明该植保素的吸收峰与黄酮类化合物的特征吸收峰相似,可能属于黄酮类化合物.
Induction of Ipomoea batatum-resistant variety “Xiangshu 6” and susceptible variety “Xinhuahua 183” were induced by Pseudomonas sp.olactoneum E.F. Smith, extracted with 90% methanol, extracted with ethyl acetate and purified with Sephadex LH -20 column chromatography to obtain four absorption peaks. The bacteriostatic activity zone of “Xiangshu 6” was detected at the second peak by the bacteriostasis test, and the third peak was the “antibacterial activity zone of the new seed 183”. Silica G254 thin-layer chromatography identification of the extract may be phytoalexin. UV and IR spectra showed that the absorption peak of the phytoalexin was similar to that of flavonoids, which may belong to flavonoids.