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目的在体外水平（invitro）建立对胶质瘤有杀伤作用的TNF－α基因／Vp16系统，研究该系统的有效性及可行性。方法用逆转录病毒载体将TNF－α基因转染人胶质瘤细胞株SHG－44，经生长抑制试验，比较转TNF－α基因前后SHG－44细胞对Vp16的敏感性。结果生长抑制试验表明，转染TNF－α基因后的SHG－44细胞对Vp16的敏感性是转基因前细胞的10倍（P＜0．01），IC50为0．8μg／ml；TNF－α基因／Vp16系统对胶质瘤的杀伤作用优于TNF－α细胞因子与Vp16的联合应用（P＜0．05），并具有良好的旁观者效应。结论在invitro水平，转TNF－α基因SHG-44细胞对Vp16的敏感性大大增加，小剂量Vp16即可达到杀伤肿瘤细胞的作用，表明TNF－α基因/Vp16系统可成为细胞因子基因联合化学药物治疗胶质瘤的有效方法。
Objective To establish a tumor necrosis factor-α gene / Vp16 system at invitro level to study the effectiveness and feasibility of this system. Methods The human glioma cell line SHG-44 was transfected with TNF-α gene by retroviral vector. The growth inhibition assay was used to compare the sensitivity of SHG-44 cells to Vp16 before and after transfection of TNF-α gene. Results The growth inhibition assay showed that SHG-44 cells transfected with TNF-α gene were 10-fold more sensitive to Vp16 than those pre-cultured with transgene (IC50 0.8μg / ml). TNF-α gene / Vp16 system is superior to glioma killing effect in combination with TNF-α cytokine and Vp16 (P <0.05), and has a good bystander effect. Conclusions At the invitro level, the sensitivity of VG-16 cells to TNF-α gene SHG-44 cells is greatly increased. A small dose of Vp16 can kill tumor cells, indicating that TNF-α gene / Vp16 system can be a cytokine gene combined with chemical drugs Effective treatment of glioma.