论文部分内容阅读
应用免疫组化ABC法，采用自制的兔抗人心肌线粒体肌酸激酶（CK－mimi）抗血清，对52例食管癌组织切片进行了检测。探讨食管癌组织中CK－mimi分布与临床病理分型的关系，旨在协助临床寻找食管癌新的诊断标志。结果显示：正常食管组织CK－mimi为阴性，52例食管癌全部为阳性，鳞癌随组织病理分级的增加而染色强度减弱。Ⅲ级鳞癌与Ⅰ级或Ⅱ级鳞癌相比，其减弱的程度均有统计学意义（P＜0．01）。提示CK－mimi可作为食管疾诊断、分级的免疫组化标志。对其防治研究具有一定的临床意义。
Using immunohistochemical ABC method, self-made rabbit anti-human myocardial mitochondrial creatine kinase (CK-mimi) antiserum was used to detect 52 cases of esophageal cancer tissue sections. To explore the relationship between the distribution of CK-mimi and clinicopathological types in esophageal cancer, aiming to help find new diagnostic markers for esophageal cancer. The results showed that: normal esophageal tissue CK-mimi was negative, 52 cases of esophageal cancer were all positive, squamous cell carcinoma with the increase in histopathological grade and staining intensity decreased. Grade III squamous cell carcinoma had a statistically significant degree of attenuation compared with Grade I or II squamous cell carcinoma (P<0.01). It is suggested that CK-mimi can be used as an immunohistochemical marker for the diagnosis and classification of esophageal diseases. Its prevention and treatment research has certain clinical significance.