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通过研究发育期大鼠脑单胺类神经递质质量比及单胺氧化酶活性,探讨环境暴露水平的六溴环十二烷对发育期大鼠的神经毒性作用。选择10μg/kg、50μg/kg、100μg/kg、300μg/kg的剂量对新生3 d大鼠进行暴露,饲养42 d后断头取脑组织,测定多巴胺(DA)、去甲肾上腺素(NE)、5-羟色胺(5-HT)的质量比及单胺氧化酶(MAO)的活性。结果表明,在10~100μg/kg的暴露剂量范围,DA质量比随暴露剂量的升高而下降,NE质量比呈现上升趋势,5-HT质量比则极显著性升高(p<0.01);与此同时,MAO的活性随剂量增加而升高,在100μg/kg剂量出现显著性升高(p<0.05)。而300μg/kg的剂量作为较高暴露水平的参照,只对DA产生显著性影响(p<0.05)。DA、5-HT、NE的质量比及MAO活性的变化可能在大鼠的中枢神经系统的发育障碍中发挥了重要作用,并且环境暴露水平的HBCD对发育期大鼠脑具有低剂量高效应的毒性反应特征。
To investigate the neurotoxic effects of HBCD on the development of rats by studying the mass ratio of monoamine neurotransmitters and monoamine oxidase activity in rat brain during development. The newborn rats were exposed to 10, 50, 100, 300μg / kg for 42 days. The brain tissue was sacrificed after 42 days. The levels of dopamine (DA), norepinephrine (NE) , Serotonin (5-HT) mass ratio and monoamine oxidase (MAO) activity. The results showed that at the dose range of 10 ~ 100μg / kg, the DA mass ratio decreased with the increase of exposure dose, the NE mass ratio showed an upward trend and the 5-HT mass ratio increased significantly (p <0.01). In the meantime, MAO activity increased with increasing dose, with a significant increase at 100 μg / kg (p <0.05). The dose of 300μg / kg, as a reference for higher exposure, had a significant effect on DA (p <0.05). The changes of mass ratio of DA, 5-HT, NE and MAO activity may play an important role in the developmental disorders of the central nervous system in rats, and the environmental exposure level of HBCD has a low dose and high effect on developing rat brain Toxic reaction characteristics.