卵母细胞冻融具有广阔的应用前景。随着冷冻技术的日趋完善,复苏率有了显著提高,但胚胎种植率及临床妊娠率仍显著低于常规体外受精,可能与纺锤体受损有关。冻融的各个环节均可能对卵母细胞的结构与功能产生影响,特别是对细胞骨架,对纺锤体及皮质颗粒的影响及是否会增加染色体非整倍体的发生尚有待进一步研究。 Oocyte freezing and thawing has broad application prospects. With the improvement of freezing technology, the recovery rate has been significantly improved, but the rate of embryo implantation and clinical pregnancy rate is still significantly lower than conventional in vitro fertilization may be related to spindle damage. All aspects of freezing and thawing may have an impact on the structure and function of oocytes, especially on the cytoskeleton, spindle and cortical granules and whether it will increase the occurrence of chromosomal aneuploidy remains to be further studied.