蕨类植物组织培养可分为以孢子为外植体的无菌播种和以孢子体为外植体进行的组织培养。本文简要介绍国内外蕨类植物组织培养研究概况,综述培养基成份、培养方式及培养条件对组织培养过程中生长发育的影响,同时介绍蕨类植物组织培养中世代转换的诱导及调控。 Fern tissue culture can be divided into spores as explants of sterile sowing and spores as explants for tissue culture. This article briefly introduced the research overview of fern tissue culture at home and abroad, reviewed the effects of medium composition, culture methods and culture conditions on the growth and development of tissue culture, and introduced the induction and regulation of the transformation of ferns in the medium.