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目的:观察七叶皂苷钠对沙土鼠脑缺血再灌注模型脑组织匀浆中内皮素(ET)和降钙素基因相关肽(CGRP)含量的影响。方法:采用结扎双侧颈总动脉缺血10 min再灌注2 h,建立沙土鼠脑缺血再灌注模型。七叶皂苷钠(10,20,40 mg·kg-1)术前3 d开始腹腔注射给药,qd,术后1 h给药1次。再灌注2 h后,放射免疫法测定脑组织ET和CGRP含量。结果:七叶皂苷钠各剂量组可降低脑组织ET水平至28.69~37.03 ng·L-1,与模型组比较有显著性差异(P<0.05或0.01),对脑组织CGRP水平则无明显影响(P>0.05)。结论:七叶皂苷钠可能通过降低脑组织ET水平,实现对沙土鼠脑缺血再灌注损伤的保护作用。
Objective: To observe the effect of sodium aescinate on the content of endothelin (ET) and calcitonin gene related peptide (CGRP) in brain homogenate of gerbil model of cerebral ischemia-reperfusion. Methods: The model of gerbil cerebral ischemia-reperfusion was established by ligating bilateral common carotid artery ischemia 10 min and reperfusion for 2 h. Sodium aescinate (10, 20, 40 mg · kg-1) was administered intraperitoneally 3 days before the operation, qd and 1 h after the operation. After 2 h of reperfusion, the contents of ET and CGRP in brain tissue were determined by radioimmunoassay. Results: The sodium aescinate could decrease the level of ET in brain tissue to 28.69 ~ 37.03 ng · L-1, which was significantly different from the model group (P <0.05 or 0.01), but had no effect on the level of CGRP (P> 0.05). Conclusion: Sodium aescinate can protect brain against ischemia-reperfusion injury in gerbils by reducing ET level of brain tissue.