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为评价螺旋藻对肠道菌群的影响,采用注射氨苄青霉素的方法,制造小鼠腹泻模型,造模成功后灌服不同剂量螺旋藻进行治疗,取不同治疗时间的粪便样品,检测双歧杆菌、乳杆菌、大肠杆菌、肠球菌、拟杆菌、产气荚膜梭菌菌群的数量,并在螺旋藻灌胃8d后,测定小鼠不同肠段各指标菌群的数量。结果表明,中高剂量组螺旋藻对腹泻模型小鼠肠道菌群有明显调整作用,有效缩短了肠道菌群由失调到平衡的时间;粪便样品同各肠段内容物所反映的菌群的变化趋势相同,即治疗恢复组的双歧杆菌、乳杆菌数量总体上高于生理盐水组,肠杆菌、肠球菌、拟杆菌、产气荚膜梭菌数量均低于生理盐水组,但取样位置不同,各指标菌群的数量明显存在差异。
In order to evaluate the effect of Spirulina on intestinal microflora, the diarrhea model of mice was made by injection of ampicillin. After the model was successfully established, different doses of Spirulina were treated by gavage, and the stool sample with different treatment time was taken to detect Bifidobacterium , Lactobacillus, Escherichia coli, Enterococcus, Bacteroidetes, Clostridium perfringens flora, and spirulina gavage 8d after the determination of the number of different mouse intestine colonies of each indicator. The results showed that spirulina in medium and high dose groups had a significant adjustment effect on intestinal flora in diarrhea model mice and effectively shortened the time from imbalance to balance of the intestinal flora; The trend of change was the same, that is to say, the number of Bifidobacterium and Lactobacillus in the treatment and recovery group was generally higher than that in the saline group. The numbers of Enterobacter, Enterococcus, Bacteroides and Clostridium perfringens were lower than those in the saline group, Different, the number of each indicator significantly different populations.