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用酶消化法分离成年雄性ＳＤ大鼠附睾头、体、尾的上皮细胞，贴壁培养。４～１１天后取贴壁细胞进行ＨＥ染色及扫描电镜观察，发现附睾头部和体部大部分为带有微绒毛的主细胞，而尾部细胞较小。并以ＰＮＰＧ为底物测定上清液内α－１．４糖苷酶的活性，结果为体部最高，尾部次之，头部最低。用硫代巴比妥酸法检测上清液中唾液酸的含量，仍以体部最高，但尾部最低。统计学处理均有显著差异。提示大鼠附睾体部可能是精子成熟的关键部位。
The epididymal, epidermal and caudal epithelial cells of adult male Sprague-Dawley rats were isolated by enzyme digestion and cultured in adherent culture. 4 to 11 days after attachment of adherent cells HE staining and scanning electron microscopy and found that most of the head and body of the epididymis with microvilli of the main cells, while the tail cells smaller. The activity of α-1,4-glucosidase in supernatant was measured by using PNPG as the substrate. The results showed that the body was the highest, the tail was the second, and the head was the lowest. Sulfobarbituric acid method to detect the content of sialic acid in the supernatant, still the highest body, but the tail of the lowest. Statistical analysis showed significant differences. Tip of the rat epididymis may be the key part of sperm maturation.