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目的:分析P120-连环蛋白(P120-catenin,P120~ctn)和E-钙粘蛋白(E-cadherin,E-cad)在口腔鳞癌(oral squamous-cell cancer,OSCC)细胞迁移和侵袭中的功能。方法:采用实时荧光定量PCR、Western blot检测HN4(人舌癌原发灶细胞)和HN12(人舌癌转移淋巴结细胞)中P120~ctn、E-cad的mRNA和蛋白表达,通过Transwell细胞侵袭及细胞迁移试验等方法检测HOK(人口腔黏膜角化上皮细胞)、HN4和HN12细胞的迁移和侵袭能力。结果:HN4细胞中P120~ctn和E-cad的mRNA和蛋白表达水平明显高于HN12,低于HOK细胞,差异均有统计学意义(P<0.05)。HN4细胞的侵袭和转移能力显著高于HOK细胞,低于HN12细胞,差异均有统计学意义(P<0.05)。结论:在OSCC细胞中P120~ctn与E-cad的表达下调,可能抑制了OSCC细胞的侵袭和转移。
OBJECTIVE: To analyze the expression of P120-catenin (P120-ctn) and E-cadherin (E-cad) in the migration and invasion of oral squamous cell carcinoma (OSCC) Features. Methods: The mRNA and protein expressions of P120 ~ ctn and E-cadherin in HN4 (human tongue cancer primary tumor cells) and HN12 (human tongue cancer metastasis lymph node cells) were detected by real-time fluorescence quantitative PCR and Western blot. Cell migration assay and other methods to detect HOK (human oral keratinized epithelial cells), HN4 and HN12 cell migration and invasion. Results: The mRNA and protein expressions of P120 ~ ctn and E-cadherin in HN4 cells were significantly higher than that in HN12 cells, but lower than that in HOK cells (P <0.05). The invasion and metastasis of HN4 cells were significantly higher than that of HOK cells, but lower than that of HN12 cells (P <0.05). Conclusion: The down-regulation of P120 ~ ctn and E-cad in OSCC cells may inhibit the invasion and metastasis of OSCC cells.