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应用银染ＰＣＲ－ＳＳＣＰ方法和免疫组织化学ＡＢＣ法，检测３０例横纹肌肉瘤（ＲＭＳ）中ｐ５３基因突变及其蛋白表达。结果显示：３０例ＲＭＳ中，７例ＳＳＣＰ阳性（２３．３％）。表明这些病例相应ＤＮＡ片段中发生了点突变，其中第５、６、７、８外显子ＳＳＣＰ阳性出现的例次分别为０、０、５、４次。ｐ５３蛋白表达率６６．７％（二者符合率５６．７％）。ｐ５３基因突变及蛋白表达与年龄、性别、肿瘤组织类型无关，而与肿瘤分化、转移和预后有关。提示，ｐ５３基因突变和蛋白表达可能是ＲＭＳ患者预后差的因素之一，可作为判断ＲＭＳ的恶性程度和预后的重要标准之一。
The p53 gene mutation and protein expression in 30 cases of rhabdomyosarcoma (RMS) were detected by silver staining PCR-SSCP method and immunohistochemistry ABC method. The results showed that in 30 cases of RMS, 7 cases were positive for SSCP (23.3%). The point mutations were found in the corresponding DNA fragments of these cases, and the occurrence of SSCP in exon 5, 6, 7, and 8 was 0, 0, 5, and 4 times, respectively. The expression rate of p53 protein was 66.7% (the coincidence rate was 56.7%). The p53 gene mutation and protein expression have nothing to do with age, gender, tumor tissue type, but related to tumor differentiation, metastasis and prognosis. It is suggested that p53 gene mutation and protein expression may be one of the factors of poor prognosis in RMS patients, and it can be used as one of the important criteria for judging the malignant degree and prognosis of RMS.