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Objective:To evaluate the significance of combined detection of LunX mRNA,carcinoembryonic antigen(CEA),neuron-specific enolase(NSE),and cytokeratin 21-1 fragment(CYFRA21-1) in clinical diagnosis of lung carcinoma.Methods:Based on the quantitative RT-PCR and chemiluminescence immunoassay,the expression levels of LunX mRNA,CEA,NSE,and CYFRA21-1 in 113 patients with lung carcinoma(case group) and 30 healthy participants(control group) were detected.Meantime,the sensitivity,specificity,and accuracy of the combination detection were also explored.Results:The positive rates of LunX mRNA in peripheral blood and CEA,NSE,and CYFRA21-1 in serum were significantly higher in case group than those in control group(χ2=17.295,16.825,19.148,and 17.450;P<0.05).There was no statistical significance when positive rate of LunX mRNA was evaluated among different pathological types(χ2=0.047,P>0.05).The positive rate of LunX mRNA in stage Ⅰ+Ⅱ,Ⅲ,and Ⅳ had a significantly increasing tendency(χ2=10.565,32.462,P<0.05).The positive rate of CYFRA21-1 was highest in squamous carcinoma(78.5%),the positive rate of NSE was highest in small cell carcinoma(86.7%),and the positive rate of CEA wag highest in lung adenocarcinoma(80.4%).The sensitivity and accuracy of the combination detection were 91.1% and 88.1%,respectively.Conclusions:The combined detection of LunX mRNA and tumor markers(TMs) including CEA,NSE,and CYFRA21-1 in peripheral blood is helpful to increase the diagnostic accuracy of lung cancer.Also,it can inform the pathological typing of lung carcinoma.
Objective: To evaluate the significance of combined detection of LunX mRNA, carcinoembryonic antigen (CEA), neuron-specific enolase (NSE), and cytokeratin 21-1 fragment (CYFRA21-1) in clinical diagnosis of lung carcinoma. Methods: Based on the quantitative RT-PCR and chemiluminescence immunoassay, the expression levels of LunX mRNA, CEA, NSE, and CYFRA21-1 in 113 patients with lung carcinoma (case group) and 30 healthy participants (control group) were detected. Meantime, the sensitivity, specificity , and accuracy of the combination detection were also explored. Results: The positive rates of LunX mRNA in peripheral blood and CEA, NSE, and CYFRA21-1 in serum were significantly higher in case group than those in control group (χ2 = 17.295, 16.825 , 19.148, and 17.450; P <0.05). Whenever the statistical significance when positive rate of LunX mRNA was classified among different pathological types (χ2 = 0.047, P> 0.05) Ⅲ, and Ⅳ had a significantly increasing tendency (χ2 = 10. 565,32.462, P <0.05). The positive rate of CYFRA21-1 was highest in squamous carcinoma (78.5%), the positive rate of NSE was highest in small cell carcinoma (86.7%), and the positive rate of CEA wag highest in lung adenocarcinoma (80.4%). The sensitivity and accuracy of the combination detection were 91.1% and 88.1% respectively. Conclusions: The combined detection of LunX mRNA and tumor markers (TMs) including CEA, NSE, and CYFRA21-1 in peripheral blood is helpful to increase the diagnostic accuracy of lung cancer. Also, it can inform the pathological typing of lung carcinoma.