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采用序贯法和霍恩法结合确定提取物LD50并确定实验剂量,选用50只昆明种小鼠随机分成空白对照组、模型对照组和3个剂量组(5.00,2.50,0.80 g/kg.BW),分别灌喂不同剂量印度人参根提取物,30 d后采血测定小鼠和肝损伤小鼠血清丙二醛含量和抗氧化酶活性.结果表明,剂量组小鼠血清MDA含量显著低于空白对照组和模型对照组;血清SOD和GSH-Px活性显著高于空白对照组;对肝损伤小鼠,剂量组MDA含量明显低于模型对照组,而SOD和GSH-Px活性则明显高于模型对照组.表明印度人参根乙醇提取物有一定抗氧化作用,对化学性肝损伤小鼠有一定保护作用.
Sequential method and Horne method were used to determine the extract LD50 and determine the experimental dose. 50 Kunming mice were randomly divided into blank control group, model control group and 3 dose groups (5.00, 2.50, 0.80 g/kg.BW ), Different doses of Indian ginseng root extract were fed, respectively, and blood was collected 30 days later to determine serum malondialdehyde levels and antioxidative enzyme activity in mice and liver injury mice. The results showed that serum MDA levels in mice dose group were significantly lower than the blank. The activity of SOD and GSH-Px in the control group and the model control group were significantly higher than those in the blank control group. The MDA content in the liver injury group was significantly lower than that in the model control group, while the SOD and GSH-Px activities were significantly higher than those in the model group. The control group showed that the ethanol extract of P. ginseng had some anti-oxidation effect and had a protective effect on mice with chemical liver injury.