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运用特殊抗体可以精确区分结缔组织肥大细胞和粘膜肥大细胞,但是,该种抗体制备极为困难,造价昂贵,一般实验室很难得到。本研究应用立体学定量分析方法,对两种类型肥大细胞的细胞核、细胞浆及分泌颗粒进行了定量分析,发现它们的核浆比、颗粒的数密度和颗粒的大小及颗粒的圆球度等方面都存在显著性差异。这些定量结果与我们对该细胞所作的组织化学反应结果一致,可作为区分这两类肥大细胞的主要形态学指标。
The use of special antibodies can accurately distinguish between connective tissue mast cells and mucosal mast cells, however, this kind of antibody is extremely difficult to prepare, costly, the general laboratory is difficult to get. In this study, quantitative analysis of nuclei, cytoplasm and secreted granules of two types of mast cells was carried out by using the quantitative analysis method of stereology. The results showed that their ratio of nucleus to pulp, the number density of granules and the size of granules and the sphericity of granules There are significant differences. These quantitative results are consistent with the results of the histochemical reactions we performed on the cells and can be used as a major morphological indicator to distinguish these two types of mast cells.