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采用HPLC法测定陈皮中橙皮甙的含量。色谱柱为C18,流动相为乙腈 - 0 4 %磷酸溶液 (2 0∶80 ) ,检测波长 2 86nm ,方法简便 ,准确 ,重现性好 ,平均回收率分别为 :98 91% ,RSD为 1 31% (n =6 )。
The content of hesperidin in orange peel was determined by HPLC. The chromatographic column was C18 and the mobile phase was acetonitrile-0.4% phosphoric acid solution (2:0:80). The detection wavelength was 286 nm. The method was simple, accurate, and reproducible. The average recoveries were 98 91% and the RSD was 1. 31% (n = 6).