论文部分内容阅读
目的:探讨中草药石菖蒲粗提物 SCP01及其纯化物 SCP02(α-细辛脑)和迷迭香抽提物 X0728对人肥大细胞的作用。方法:用膜片钳技术全细胞记录人肥大细胞膜 ATP 激活的 P2X_7受体的电流。结果:40μg/mL SCP01抑制 P2X_7电流(27.6±2.0)%,而同样浓度的 SCP02抑制(29.5±2.2)%(-100 mV 电位下),笔者还比较了商业用α-细辛脑的效应,42 μg/mL 可抑制 P2X_7电流(52.2±2.0)%。相反,40μg/mL X0728激活 P2X_7电流(28.6±2.8)%,所有这些作用都是电压依赖性的。结论:α-细辛脑对 P2X_7的抑制将阻滞胞内钙的增加,从而可以解释抑制神经元死亡的原因。而 X0728刺激 P2X_7的药理学效应尚需进一步研究。
Objective: To investigate the effect of SCP01 and its purified SCP02 (α-asarone) and rosemary extract X0728 on human mast cells. METHODS: Whole-cell patch clamp technique was used to record the current of the mast cell membrane ATP-activated P2X_7 receptor. Results: 40μg/mL SCP01 inhibited P2X_7 current (27.6±2.0)%, while the same concentration of SCP02 inhibited (29.5±2.2)% (under 100 mV potential), the author also compared the effects of commercial α-asarone, 42 μg/mL can inhibit P2X_7 current (52.2 ± 2.0)%. In contrast, 40 μg/mL X0728 activated P2X_7 current (28.6±2.8)%, all of these effects were voltage-dependent. Conclusion: Inhibition of P2X_7 by α-asarone will block the increase in intracellular calcium, which may explain the reason for inhibition of neuronal death. However, the pharmacological effects of X0728-stimulated P2X_7 still need further study.