目前用于测定白喉菌苗效力的方法受到这种或那种限制。作者发展了一种评价人血清中白喉和破伤风抗毒素的方法,称为毒素结合抑制试验(TOBI),此法基于毒素同抗毒素结合的抑制作用。方法是,将血清2倍系列稀释液40μl同80μl白喉毒素(0.01Lf/ml,磷酸缓冲液(PBS),pH7.2)于微量滴定板孔中混合,摇匀,37℃孵育过夜。次日取100μl The methods currently used to determine the efficacy of diphtheria vaccine are limited to this or that. The authors developed a method for evaluating diphtheria and tetanus antitoxin in human serum called toxin binding inhibition test (TOBI), which is based on the inhibition of toxin binding to antitoxin. The method is as follows: Mix 40μl of 2 times serial dilutions of serum with 80μl diphtheria toxin (0.01Lf / ml, phosphate buffered saline (PBS), pH7.2) in microtiter plate wells, shake well and incubate overnight at 37 ℃. The next day to take 100μl