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目的 研究幽门螺杆菌 (Hp)全菌抗原的免疫原性及Anti Hp IgY的预防和治疗作用。方法 用超声Hp波粉碎的Hp抗原免疫产蛋母鸡 ,通过IHA和ELISA间接法检测母鸡的免疫应答。用SDS PAGE分析Hp全菌抗原 ,并用抗Hp IgY做免疫印迹。结果 母鸡免疫成功率为 10 0 %。初免 45d后 ,抗Hp IgYIHA效价为 1∶10 2 4,ELISA效价超过 1∶45 0 0 0。Hp抗原的SDS PAGE显示有 2 6条蛋白染色带 ,其中主带有 10条 ,免疫印迹表明Hp的超声粉碎物特异性抗原蛋白带相对分子质量为 70 0 0 0、6 6 0 0 0、42 0 0 0、3430 0、2 95 0 0、2 70 0、185 0 0。结论 本研究为工业化制备抗Hp IgY奠定了基础
Objective To study the immunogenicity of Helicobacter pylori (Hp) whole bacterial antigen and the preventive and therapeutic effects of Anti Hp IgY. Methods The laying hens were immunized with Hp antigen pulsed with ultrasonic Hp wave and the immune response of the hens was detected by IHA and ELISA. Hp whole bacterial antigen was analyzed by SDS PAGE and immunoblotted with anti-Hp IgY. Results The hen immune success rate was 100%. After 45 days, the titer of anti-Hp IgYIHA was 1:10 2 4 and the ELISA titer was over 1:45 0 0 0. Hp antigen SDS PAGE showed 26 protein bands, of which the main with 10, Western blot showed that the ultrasonic pulverized Hp antigen-specific protein with a molecular weight of 70 0 0 0,6 6 0 0 0,42 0 0 0,3430 0,2 95 0 0,2 70 0,185 0 0. Conclusion This study laid the foundation for the industrial preparation of anti-Hp IgY