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【目的】研究狗肝菜多糖(DCP)对抗结核药物致肝损伤的保护作用及机制。【方法】将60只小鼠随机分为6组,即正常对照组、模型组、葡醛内酯组(剂量为200 mg/kg)及狗肝菜多糖高、中、低剂量组(剂量分别为600、400、200 mg/kg)。除正常对照组外,其他各组均灌胃异烟肼和利福平各100 mg/kg造模,同时给予治疗药物,每天1次。在实验的第30天,取血和肝组织,采用生物化学法检测血清中丙氨酸转氨酶(ALT)、门冬氨酸转氨酶(AST)、碱性磷酸酶(AKP)以及微粒体一氧化氮(NO)生化指标;采用酶联免疫吸附法检测肝组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)含量;应用苏木精—伊红(HE)染色进行肝组织病理学检查。【结果】狗肝菜多糖可减轻抗结核药物引起的小鼠肝组织病理损害,减少炎性细胞,对肝损伤小鼠血清ALT、AST、AKP活性及肝组织中TNF-α、IL-6、NO含量具有抑制或降低作用(P<0.05或P<0.01)。【结论】狗肝菜多糖对抗结核药物致肝损伤的保护作用可能与其介导的抗炎作用有关。
【Objective】 To study the protective effect and mechanism of hepatopancreas polysaccharide (DCP) on liver injury induced by antituberculosis drugs. 【Methods】 Sixty mice were randomly divided into six groups: normal control group, model group, glucurolactone group (200 mg / kg) and high, medium and low doses of DPC , 400, 200 mg / kg). In addition to the normal control group, the other groups were given 100 mg / kg of isoniazid and rifampicin each model, while giving the treatment drug once a day. On the 30th day of the experiment, blood and liver tissues were taken for detection of serum ALT, AST, AKP and microsomal nitric oxide (NO). The levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were detected by enzyme-linked immunosorbent assay (ELISA) Liver histopathology examination. 【Result】 Apocynum venetum polysaccharide could reduce pathological damage of liver tissue and decrease inflammatory cells in mice induced by antituberculosis drugs. The activity of ALT, AST and AKP in serum and the contents of TNF-α, IL-6 and NO in liver tissues of mice with liver injury Had inhibitory or reducing effects (P <0.05 or P <0.01). 【Conclusion】 The protective effect of Hepatic Giralum polysaccharides on the hepatic injury induced by antituberculosis drugs may be related to its anti-inflammatory effects.