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研究蒲公英提取物对急性肺损伤(ALI)小鼠炎症介质NO和PGE2的限速酶iNOS和COX-2 mRNA及核转录因子NF-κB(p65)蛋白表达的影响,初步探讨其对ALI小鼠的保护作用机制。采用逆转录聚合酶链式反应(RT-PCR)法检测iNOS和COX-2的mRNA表达,Western-blot检测NF-κB(p65)的表达。结果表明蒲公英提取物抑制了ALI小鼠肺组织中iNOS、COX-2 mRNA及NF-κB蛋白的表达,分析其对ALI小鼠的保护作用可能通过此途径完成。
To study the effect of dandelion extract on the expression of iNOS, COX-2 mRNA and NF-κB (p65) in inflammatory mediators NO and PGE2 in acute lung injury (ALI) mice, Protection mechanism. The mRNA expressions of iNOS and COX-2 were detected by reverse transcription-polymerase chain reaction (RT-PCR) and the expression of NF-κB (p65) was detected by Western-blot. The results showed that dandelion extract inhibited the expression of iNOS, COX-2 mRNA and NF-κB in the lung tissue of ALI mice. The protective effect of dandelion extract on ALI mice may be accomplished through this pathway.