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本文报道用甲型流感病毒冷适应减毒株caA/川崎/9/86(H1N1)和caA/洛杉矶(H3N2)免疫儿童的结果,并对二价疫苗中毒株间的干扰作了初步探讨.将182名6～18月龄血清阴性的儿童以双盲法分为4组:H1N1组、H3N2组、H1N1及H3N2二价疫苗组和接种鸡胚尿囊液的对照组.试验用疫苗滴度均为10~(6.2)半数组织培养感染量(TCID_(50)).疫苗接种后密切观察1周:疫苗组儿童未见明显不良反应,轻微呼吸道症状及低热与对照组无显著性差异.鼻洗液的流感病毒分离率:免疫后6天H1N1组为16%,H3N2组为36%,二价疫苗组为55%;11天时H1N1组为0%,H3N2组为2%,二价疫苗组为8%.全部分离物经检验仍保留减毒特性而无毒力恢复迹象.疫苗接种后4周,H3N2组抗体阳转率达90%以上,抗体几何平均滴度(GMT)为7.2,均显著高于H1N1组.二价疫苗组的H3N2抗体阳转率与单价疫苗组无明显差别,但H1N1抗体仅为31%,明显低于单价疫苗组(83%).
In this paper, the results of immunization of children with cold-adapted attenuated strains of influenza A virus caA / Kawasaki / 9/86 (H1N1) and caA / Los Angeles (H3N2) were reported in this paper. 182 children aged 6 to 18 months who were seronegative were divided into 4 groups by H1N1 group, H3N2 group, H1N1 and H3N2 bivalent vaccine group and control group inoculated with chick embryo allantoic fluid (TCID_ (50)) in 10 ~ (6.2) half of the tissue culture.The close observation for 1 week after vaccination showed no obvious adverse reactions in the vaccine group, mild respiratory symptoms and low fever were not significantly different from the control group. Fluid influenza virus isolation rate: 6 days after immunization H1N1 group was 16%, 36% of the H3N2 group, 55% of the bivalent vaccine group; H1N1 group was 0% on day 11, H3N2 group was 2%, bivalent vaccine group was 8% .All the isolates still retain the attenuated character without any sign of virulence recovery.After 4 weeks of vaccination, the positive rate of antibody in H3N2 group was over 90% and the geometric mean antibody titer (GMT) was 7.2, both of which were significant Which was higher than H1N1 group.The H3N2 antibody positive rate in the bivalent vaccine group was not significantly different from the monovalent vaccine group, but the H1N1 antibody was only 31%, significantly lower than the monovalent vaccine group (83%).