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目的研究芩连片高效液相色谱指纹图谱,建立整体特征的质量评价方法。方法采用DAD-ELSD串联检测技术,Zorbax色谱柱,以乙腈-1.5%冰醋酸为流动相,梯度洗脱,检测波长为280nm。结果本色谱条件下芩连片各成分得到了较好的分离,标准指纹图谱均由10个特征峰组成。结论所建立的HPLC指纹图谱表达了芩连片可测组分的整体特征。具有重复性好、特征性强、方法简便等特点,可用于芩连片的质量评价。
Objective To study the HPLC fingerprint of Qinlian tablets and to establish the quality evaluation method of the whole features. Methods DAD-ELSD tandem detection was used. The mobile phase was acetonitrile-1.5% glacial acetic acid. The mobile phase was Zorbax. The detection wavelength was 280 nm. The results of the chromatographic conditions Qinlian pieces of each component has been better separation, the standard fingerprint consists of 10 characteristic peaks. Conclusion The established HPLC fingerprinting expresses the overall characteristics of the measurable components of Qinlian tablets. With good repeatability, strong characteristic, simple and convenient method, it can be used for the quality evaluation of Qinlian tablets.