本文介绍关于微量血液淋巴细胞转化的放射性测量法:指尖采血0.05毫升,全血培养37℃,94小时,收获细胞前16小时加入氚-胸腺嘧啶核苷(~3H-TdR)0.6微居里。培养结束时洗脱培养液中游离的放射性,然后用三氯醋酸提取细胞中的DNA,用液体闪烁计数器测定其中所含的~3H-TdR 的放射性,即可反映淋巴细胞转化。在125人次的测定中,初步得出输血者淋巴细胞转化的正常值,并发现某些血液病人及肿瘤病人的淋巴细胞转化能力明显降低,电离辐射对它亦有抑制作用。 This article describes the radiometric measurement of lymphocyte transformation in micronutrients: finger-tip blood collection at 0.05 ml, whole blood culture at 37°C for 94 hours, and addition of technetium-thymidine (~3H-TdR) 0.6 μC . At the end of the culture, the free radioactivity in the culture medium was eluted, and the DNA in the cells was extracted with trichloroacetic acid. The radioactivity of ~3H-TdR contained therein was measured by a liquid scintillation counter to reflect the lymphocyte transformation. In the 125-times determination, the normal values ​​of lymphocyte transformation in the donor were initially found, and it was found that the lymphocyte transformation ability of some blood patients and tumor patients was significantly reduced, and ionizing radiation also had an inhibitory effect on it.