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小黄黝鱼(Micropercops swinhonis)是广布于中国长江及北方各水系的一种小型淡水虾虎鱼类。本研究采集了来自松花江(哈尔滨)、辽河(沈阳)、海河(北京)、黄河下游(濮阳)、高邮湖、长江水系(邵阳资水、洪湖、荆州、靖江市、巢湖、太湖、郎溪、洞庭湖)和云南腾冲的88个样品。通过分析线粒体细胞色素b(Cyt b)和控制区D-loop基因序列的变异研究小黄黝鱼不同地理种群的相互关系,并探讨其遗传结构和物种分化。Cyt b和D-loop的序列串联共得到63个单倍型,133个变异位点。AMOVA、SAMOVA、网络图以及贝叶斯建树分析结果都支持将其分为邵阳资水种群(A种群)、太湖种群(B种群)、其他地区种群(C种群)3个差异较大的分支,其中C种群又大致可以分3个子种群。错配分布分析表明靖江(Fu’s FS:-4.119,P=0.009)和洪湖(Fu’s FS:-2.814,P=0.016)两地的种群历史上发生过种群扩张。
Micropercops swinhonis is a small freshwater goby widely distributed in the Yangtze River and Northern Rivers of China. This study collected from the Songhua River (Harbin), Liaohe (Shenyang), Haihe (Beijing), the lower reaches of the Yellow River (Puyang), Gaoyou Lake, the Yangtze River water system (Shaoyang Zishui, Honghu, Jingzhou, Jingjiang City, Chaohu, Taihu Lake Langxi , Dongting Lake) and Yunnan Tengchong 88 samples. The relationship between mitochondrial cytochrome b (Cyt b) and control region D-loop gene sequence was analyzed to study the genetic relationship among different geographical populations and their genetic structure and species differentiation. A total of 63 haplotypes and 133 mutated sites were obtained by the tandem sequence of Cyt b and D-loop. AMOVA, SAMOVA, network diagram and Bayesian tree analysis results are divided into three widely divergent branches: Shaoyang Zishui population (A population), Taihu Lake population (B population) and other areas (C population) Among them, C population can be roughly divided into 3 sub-populations. Mismatch distribution analysis showed that population expansion occurred in the population history of Jingjiang (Fu’s FS: -4.119, P = 0.009) and Honghu (Fu’s FS: -2.814, P = 0.016).