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目的 研究极低频电磁场是否影响细胞内钙离子浓度 ( [Ca2 +]i)。方法 Fura 2负载HepG2细胞分别在 1.5 5mT(均值 )、16Hz脉冲磁场处理 6 0min ,30 0mT、2Hz旋转磁场处理 5min后 ,用荧光光度计检测 [Ca2 +]i;实时检测 0 .9mT(有效值 )、16Hz正弦磁场对 [Ca2 +]i的影响。结果 在 1.5 5mT、16Hz脉冲磁场处理后 ,对照组和处理组R值 (F34 0nm F380nm )分别为 2 .4 5 19± 0 .2 378、2 .5 2 6 6± 0 .2 915 ,30 0mT、2Hz旋转磁场处理后R值分别为 1.36 5 0± 0 .0 6 2 6、1.36 0 2± 0 .0 771。 0 .9mT、16Hz正弦磁场处理下R值数据点拟合趋势线斜率比 [r(50 1～ 1 0 0 0 ) r(0～ 50 0 ) ]分别为 1.12 13± 0 .4 5 5 9、1.0 72 7± 0 .1971,截距之比 [b(50 1～ 1 0 0 0 ) b(0～ 50 0 ) ]分别为 0 .9912± 0 .0 0 98、0 .9979± 0 .0 0 6 0 ,差异均无显著性 (P >0 .0 5 )。结论 在本实验条件以及所采用的分析方法下未发现磁场对HepG2的 [Ca2 +]i产生影响
Objective To investigate whether ELF electromagnetic fields affect intracellular calcium concentration ([Ca2 +] i). Methods Fura 2 loaded HepG2 cells were treated with 1.5 5 mT (mean), 16 Hz pulsed magnetic field for 60 min, 30 0 mT, 2 Hz rotating magnetic field for 5 min, respectively. [Ca 2+] i was detected by fluorophotometer; real- ), 16Hz sine magnetic field [Ca2 +] i. Results The R values (F34 0nm F380nm) of the control group and the treated group after treatment with 1.55mT and 16Hz pulsed magnetic fields were respectively 2.45 19 ± 0.237 8,2.52 6 6 ± 0.2915 and 30 0mT , And the values of R after 2Hz rotating magnetic field treatment are 1.36 5 0 ± 0 .0 6 2 6,1.36 0 2 ± 0 .0 771 respectively. The slope ratios [r (50 1 ~ 1 0 0 0) r (0 ~ 50 0)] of fitting trend line of R value data points under the sinusoidal magnetic field of 0 .9mT and 16Hz were 1.12 13 ± 0.459, 1.072 7 ± 0.1971, the intercept ratio [b (50 1 ~ 1 0 0 0) b (0 ~ 50 0)] was 0.9912 ± 0.0898, 0.9979 ± 0 .0 0 6 0, the difference was not significant (P> 0.05). Conclusions The influence of magnetic field on [Ca2 +] i in HepG2 was not observed under the experimental conditions and analytical methods used