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目的 :建立卵胞浆内单精子注射技术 ( ICSI)前人精子激活卵细胞相关因子检测方法。方法 :选用 2 0～ 2 5 g昆明雌性白鼠 ,超排卵取卵细胞 ,上游法优选人正常精子显微注射 ,对实验中一些环节进行观察比较。结果 :显微注射针内径 5～ 7μm较适合人精子与鼠卵细胞之间的注射 ,小鼠卵细胞体外培养 2 h左右进行显微注射效果较好 ,显微注射人精子的鼠卵细胞激活率与实验对照组、空白对照组比较 ,前者有较高的激活率 ,激活过程不易受操作程序及自发激活的影响 ,与人卵细胞接近。结论 :此模型可用于 ICSI前人精子激活能力的检测
OBJECTIVE: To establish a method for the detection of related factors of activated spermatozoa of ovary by intracytoplasmic sperm injection (ICSI). Methods: Female Kunming mice (20 ~ 25 g) were selected for ovulation and oocytes were taken from superovulation. The normal human sperm was selected by microinjection of the upstream method, and some aspects of the experiment were observed and compared. Results: The injection diameter of 5 ~ 7μm microinjection needle is more suitable for injection between human sperm and mouse egg cells. Mouse egg cells cultured in vitro for about 2 hours are better for microinjection. The rate of mouse egg cell activation in microinjection of human sperm and experimental The control group, the blank control group, the former has a higher activation rate, the activation process is not easily affected by the operating procedures and spontaneous activation, and human egg cells close. Conclusion: This model can be used to detect sperm activation in ICSI