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目的探讨氟斑牙的发病机制及褪黑素是否对氟斑牙的发生有拮抗作用。方法 2009年3—10月于中国医科大学公共卫生学院将40只Wistar大鼠随机分为6组,包括阴性对照组、低氟组、高氟组、低氟+褪黑素组、高氟+褪黑素组。建立氟斑牙动物模型,制作切片后行HE染色,光镜下观察各组大鼠切牙成釉细胞形态。结果单纯给氟组大鼠。出现牙面粗糙、棕白色相间横纹、白垩色改变,高氟组大鼠的氟斑牙改变较低氟组的改变典型;成釉细胞扭曲变形,正常的高柱状形态丧失,胞内出现空泡等。注射褪黑素组与单纯给氟组的切牙一般状态及成釉细胞形态、排列未见明显差异。结论氟对大鼠切牙的成釉细胞有毒性效应,饮水氟含量高所致氟斑牙症状加重。褪黑素对氟斑牙的发生是否有拮抗作用有待进一步研究。
Objective To investigate the pathogenesis of dental fluorosis and whether melatonin has an antagonistic effect on the occurrence of dental fluorosis. Methods From March to October, 2009, 40 Wistar rats were randomly divided into 6 groups at the School of Public Health, China Medical University. They included negative control group, low fluoride group, high fluoride group, low fluoride + melatonin group, high fluoride + Melatonin group. The animal model of dental fluorosis was established. The sections were stained and HE stained. The morphology of ameloblasts in each group was observed under light microscope. The results were simple to fluoride rats. The appearance of coarse teeth, brownish white phase stripes and chalky change, the change of dental fluorosis in high fluoride group is typical of the low fluorine group. The ameloblasts distort, the normal high columnar shape is lost and the intracellular empty Bubble and so on. Injection of melatonin group and simple fluoride group incisor general state and ameloblast morphology, arrangement no significant difference. Conclusion Fluoride has a toxic effect on ameloblasts in rat incisors, and the dental fluorosis symptoms are aggravated by high fluoride content in drinking water. Melatonin on the occurrence of dental fluorosis antagonism remains to be further studied.