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为了研究小麦类钙调素基因的功能和在植物逆境响应中的分子机制,以电子克隆结合RT-PCR的方法,在小麦中克隆到一个类钙调素基因TaCML79。该基因的开放阅读框长度为588bp,编码的蛋白长度为195个氨基酸,推测的分子量为20.45kDa,等电点为4.6,属于酸性蛋白,具有2个典型的和一个不完整的EF-hand结构域。多序列比对和系统进化树分析表明,TaCML79与野生稻的ObCML35和水稻的OsCML10亲缘关系较近,相似性分别为84.6%和86.8%。该基因的表达在根和地上部分受到热激、NaCl、渗透和冷胁迫的诱导或抑制,初步推断该基因可能与植物抗逆有密切的关系。
In order to study the function of wheat calmodulin gene and its molecular mechanism in response to plant stress, a calmodulin-like gene, TaCML79, was cloned from wheat by electronic cloning combined with RT-PCR. The open reading frame of this gene is 588 bp in length and encodes a protein of 195 amino acids in length with a predicted molecular mass of 20.45 kDa and an isoelectric point of 4.6 which is acidic and has two typical and an incomplete EF-hand structure area. Multiple sequence alignment and phylogenetic tree analysis showed that TaCML79 has a close genetic relationship with both ObCML35 of wild rice and OsCML10 of rice with similarities of 84.6% and 86.8%, respectively. The expression of this gene was induced or inhibited by heat shock, NaCl, osmotic stress and cold stress in the roots and shoots. It was preliminarily concluded that this gene may be closely related to the plant resistance to stress.